Journal: Scientific reports
Article Title: Clusterin mediates hydroquinone-induced cytotoxic responses in HL-60 differentiated cells.
doi: 10.1038/s41598-024-82140-0
Figure Lengend Snippet: Fig. 4. CLU protein concentration in HL-60 cells exposed to different concentrations of HQ. CLU protein levels were quantified using ELISA after treatment with HQ at concentrations of 0, 10, 25, and 50 µmol/L (n = 3 per group). The error bars represent SD, and the mean is shown as horizontal bars. The control group (0 µmol/L HQ) exhibited the highest CLU concentration (19.05 ± 2.32 ng/mL), while HQ-treated groups showed a progressive decrease: 14.45 ± 1.23 ng/mL at 10 µmol/L, 9.43 ± 0.40 ng/mL at 25 µmol/L, and 9.46 ± 0.67 ng/mL at 50 µmol/L. Significant differences were determined by ANOVA followed by Tukey post hoc test (*p < 0.05, **p < 0.01, ***p < 0.001, nsp > 0.05), ns: not significant.
Article Snippet: The Human Clusterin Enzymelinked immunosorbent assay (ELISA) Kit was obtained from Boster Biological Technology (China).
Techniques: Protein Concentration, Enzyme-linked Immunosorbent Assay, Control, Concentration Assay